Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2)

Uguru, Gabriel C, Mondhe, Madhav, Goh, Shan, Hesketh, Andrew, Bibb, Mervyn J, Good, Liam and Stach, James E M (2013) Synthetic RNA Silencing of Actinorhodin Biosynthesis in Streptomyces coelicolor A3(2). PLoS ONE, 8 (6). e67509. ISSN 1932-6203

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We demonstrate the first application of synthetic RNA gene silencers in Streptomyces coelicolor A3(2). Peptide nucleic acid and expressed antisense RNA silencers successfully inhibited actinorhodin production. Synthetic RNA silencing was target-specific and is a new tool for gene regulation and metabolic engineering studies in Streptomyces.